Uses of Disodium Hydrogen Phosphate.

Buffer Solutions:

Disodium hydrogen phosphate is commonly used as a component in buffer solutions, especially those used in biological and biochemical experiments. Buffer solutions help maintain a constant pH level, which is crucial for many reactions and experiments.

DNA and Protein Electrophoresis:

In molecular biology and biochemistry laboratories, disodium hydrogen phosphate is used in gel electrophoresis procedures for separating DNA or proteins based on their size and charge. It helps in the preparation of running buffers for these procedures.

Cell Culture Media:

In cell culture applications, disodium hydrogen phosphate can be included in the formulation of cell culture media to maintain a stable pH environment for cell growth and proliferation.

Analytical Chemistry:

Disodium hydrogen phosphate is used as a reagent in various analytical chemistry techniques, such as spectrophotometry and chromatography, for sample preparation, calibration, and standardization.

Titration:

In titration experiments, disodium hydrogen phosphate can be used as a standard solution or as a titrant to determine the concentration of acids or bases in a given solution.

Phosphate Analysis:

It is also used in the analysis of phosphate levels in environmental samples, such as water and soil, as well as in biological samples, to assess phosphate content or to study phosphate metabolism.

Protein Purification:

Disodium hydrogen phosphate can be utilized in protein purification procedures, such as ion exchange chromatography, as a component of the buffer system for protein elution and separation.