Uses of Trisodium Orthophosphate

Buffer Solution:

TSP is used in the preparation of buffer solutions, especially those with a pH around 12. Its ability to maintain a stable pH makes it valuable in biochemical and molecular biology experiments.

Staining Procedures:

In histology and cytology, TSP may be used as a component in staining procedures, particularly for special stains where its unique properties are advantageous.

Protein and Enzyme Studies:

TSP is utilized in protein and enzyme studies as a component of certain buffers used for protein extraction, purification, and enzymatic assays. Its ability to solubilize proteins makes it useful in these applications.

Phosphate Source:

TSP serves as a source of phosphate ions in various chemical reactions and analytical procedures. Phosphate is involved in numerous biochemical processes, making TSP valuable in studying these processes.

DNA Extraction:

In molecular biology, TSP may be used in DNA extraction protocols as a component of lysis buffers to disrupt cell membranes and release DNA from cells.

Electrophoresis:

TSP is sometimes included in electrophoresis running buffers to help maintain the desired pH and ionic strength of the buffer solution during gel electrophoresis experiments.

Corrosion Studies:

TSP is used in corrosion studies to assess the effectiveness of corrosion inhibitors and to study the corrosion behavior of various materials in different environments.

Analytical Chemistry:

TSP may be used as a reagent or standard in certain analytical chemistry techniques, such as spectrophotometry or titration methods.